Protein kinase C activity in peripheral blood Tlymphocytes and its significance in patients with allergic rhinitis
- VernacularTitle:变应性鼻炎外周血T淋巴细胞蛋白激酶C活性检测
- Author:
Shuqi QIU
;
Xianhai ZENG
;
Lingguo MA
;
Juanjuan LI
;
Xiaoli JIA
- Publication Type:Journal Article
- Keywords:
Protein Kinase C;
Interleukin-4;
Interleukin-5;
Rhinitis, Allergic, Seasonal
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the protein kinase C (PKC) activity in peripheral blood T lymphocytes and its significance in patients with allergic rhinitis (AR) and the relationship between PKC activity and Th2 cytokines, interleukin 4 (IL-4) and interleukin 5 (IL-5) . METHODS Twenty seasona allergic rhinitis patients and 20 normal control persons participated in the study. T lymphocytes isolated and purified from blood of each person were divided into 2 groups:stimulated with and without phorbol 12-myristate 13-acetate (PMA) . The total PKC activity was detected by non-radioactive assay. The expression of IL-4 and IL-5 protein in supernatants was measured with ELISA RESULTS PKC activity, the expression of IL-4 and IL-5 protein in supernatants in AR T lymphocyte stimulated with PMA were significantly higher than those of AR T lymphocyte stimulated without PMA and those of norma (P
