Prostaglandin E_2 inhibits transforming growth factor-?_1 triggered lung fibroblast-myofibroblast transdifferentiation and collagen Ⅰ production in vitro
- VernacularTitle:前列腺素E_2抑制转化生长因子-?_1诱导的人胚肺成纤维细胞转分化及胶原合成
- Author:
Xiang LONG
;
Shengdao XIONG
;
Weining XIONG
;
Yongjian XU
;
Zhenxiang ZHANG
;
Yong CAO
;
Jun CHEN
;
Xiaohong XU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Fibroblasts;
Prostaglandins E;
Connective tissue growth factor;
Collagen type Ⅰ
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of prostaglandin E2 on transforming growth factor-?1(TGF-?1)triggered human lung fetus fibroblast(HLF)transdifferentiation and connective tissue growth factor(CTGF),collagen type I(COLⅠ)expression.METHODS:HLFs were treated with TGF-?1,the cells underwent phenotypic change to myofibroblast.The marker of myofibroblast-?-smooth muscle actin(?-SMA)was detected by immunofluorescence.The ?-SMA content was measured by Western blotting.The changes in CTGF and COL Ⅰ at transcription levels were estimated by RT-PCR method.CTGF protein expression was evaluated by immunocytochemical.Cell culture medium hydroxyproline amount was measured by colormetric assay.RESULTS:PGE2 blocked TGF-?1 induced ?-SMA positive myofibroblast transformation(P
