Effect of F10 gene on activities of transcriptional factor NF-?B and AP1
- VernacularTitle:F10基因对转录因子NF-?B、AP1活性的影响
- Author:
Xiaomin CAO
;
Zhanjun PANG
;
Song QUAN
;
Fuqi XING
- Publication Type:Journal Article
- Keywords:
Hydatidiform mole;
Genes,F10;
NF-kappa B;
Transcription factor AP-1
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the influence of F10 on the activities of transcription factor NF-?B and AP1 in A549 cells.METHODS:The luciferase report plasmids of NF-?B-Luc,AP1-Luc and F10 gene were introduced into A549 cells and the luciferase activity was detected.The DNA binding activities of AP1 and NF-?B in the cells were measured by the electrophoretic mobility shift assay(EMSA).RESULTS:The luciferase activity in F10+ transfection group decreased 30% and increased 2-fold respectively 48 h after transfected with the luciferase report plasmid of NF-?B-Luc and AP1-Luc in A549 cells.The DNA binding activity decreased 49% and increased 23%,respectively.CONCLUSION:F10 gene up-regulates the transcription activity of AP1 and down-regulates the NF-?B in A549 cells.
