Effect of dominant negative Ⅰ?B? transfection on NF-?B pathway and cell motility in breast cancer
- VernacularTitle:显性负性Ⅰ?B?对乳腺癌细胞株核因子?B通路和运动迁移的影响
- Author:
Hong WANG
;
Junping ZHANG
;
Wenpeng ZHOU
;
Houjun CAO
;
Yong FANG
;
Jinmin WU
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
NF-kappa B;
Cell transfection;
Cell movement
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect on NF-?B pathway and cell motility in breast cancer cell lines after transfection of dominant negative I?B? plasmid.METHODS:After stable transfection of mutant I?B? plasmid into highly metastatic breast cancer lines MDA-MB-231 and MDA-MB-435,we detected NF-?B binding activity by EMSA,cell growth ability by cell growth curve,colony forming test,and cell motility by millicell-PCF chamber.RESULTS:Constitutive activities in MDA-MB-231 and MDA-MB-435 were observed.Stable transfection of a dominant negative Ⅰ?B? resulted in downregulation of NF-?B binding activity,thus inhibited cell mobility without significant effect on cell growth.CONCLUSION:Cell migration ability is inhibited in highly invasive breast cancer cells by inhibition of NF-?B pathway in vitro.
