Expression of BRMSI in different metastasic breast cancer cells and its relation to HDAC activity
- VernacularTitle:BRMS1在不同转移性乳腺癌细胞中的表达及其与HDAC活性的关系
- Author:
Yangrong XU
;
Qingmo LIANG
;
Yuefeng TANG
;
Jian LIAO
- Publication Type:Journal Article
- Keywords:
Breast Neoplasms/pathol;
BRMS1;
HDAC
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of BRMS1 in different metastatic breast cancer cells and its relation to HDAC activity.Methods The gene expression of BRMS1 in 3 types breast cancer cell lines(non-metastasis MCF-7(A cell line),low metastasis MDA-MB-453(B cell line)and high metastasis MDA-MB-231(C cell line)) was determied by RT-PCR technique;the protein expression of BRMS1 was measured with Western blot technique and HDAC activity by enzyme linked immunosorbent assay.Results(1) Gene expressive ratio of BRMS1 was 3.1∶2.0∶1.0 in the A、B、C cell lines,respectively.The gene expression of BRMS1 in the C cell line decreased 210% compared to A cell line,and expression of BRMS1 was markedly reduced as the degree of metastasis increased(P
