Determination of Thiomersal in Hepatitis B Vaccine by Flow Injection-Hydride Generation Atomic Absorption Spectrometry
- VernacularTitle:流动注射氢化物发生-原子吸收法测定乙肝疫苗中硫柳汞的含量
- Author:
Xiaohua YANG
;
Yinghua LIU
;
Yuchun WANG
- Publication Type:Journal Article
- Keywords:
Hydride generation atomic absorption spectrometry;
Hepatitis B vaccine;
Thiomersal;
Content
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for the determination of the content of thiomersal in hepatitis B vaccine. METHODS: The content of thiomersal in hepatitis B vaccine was determined by flow injection-hydride generation atomic absorption spectrometry. The samples were treated by reflux digestion method with 4% sulphuric acid as medium,0.5% potassium borohydride as reducing agent,and 0.5% sulphuric acid as supporting liquid. RESULTS: The linear range of thiomersal was 5.0～100 ?g?L-1,with an average recovery rate of 100.6%(RSD=0.89%) and the lowest detection limit of 1.00 ?g?L-1. CONCLUSION: The method is simple,rapid and sensitive,and suitable for the determination of the content of thiomersal in hepatitis B vaccine.
