Relationship between potassium channel and ADAR1 in Hep-2 cell line
- VernacularTitle:人喉癌Hep-2细胞膜钾离子通道与RNA编辑酶1的相关性
- Author:
Wenfa YU
;
Yulin ZHAO
;
Kai WANG
;
Mingmin DONG
- Publication Type:Journal Article
- Keywords:
Laryngeal Neoplasms;
Potassium Channels, Voltage-Gated;
Path-Clamp Techniques;
Reverse Transcriptase Polymerase Chain Reactino;
RNA Editing;
Enzymes
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the relationship between potassium channel and ADAR1 in Hep-2 cell line. METHODS The potassium current was recorded by the whole-cell recording technique of perforated membrane clamp. Using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) , the expressions of ADAR1 mRNA in the Hep-2cell line were detected. RESULTS The similar membrane current was observed when cells were held at -40 mV and test potentials ranged from -80 mV to +80 mV. The current exhibited properties of voltage-dependent, outward rectification. It exhibited complete activation after alatency of 25ms and no or little inactivation over the 800ms voltage pulse. It could be blocked by potassium channel blockers TEA. The relative intensities of ADAR1 mRNA of t he Hep-2 cell line were different before and after its potassium channel was blocked. CONCLUSION Delayed rectifier potassium channel exist in human laryngeal carcinoma cell line Hep-2.The potassium channel is voltage-dependent. There is a correlation between the potassium channel and ADAR1 mRNA in Hep-2 cell line.
