Evaluation on the applicability of tryptase and IgE assay to postmortem diagnosis of the sudden death from hypersensitivity
- VernacularTitle:类胰蛋白酶和IgE测定在过敏性猝死诊断中的应用研究
- Author:
Cairong GAO
;
Xiangjie GUO
;
Junhong SUN
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Sudden death from hypersensitivity;
MCT;
IgE;
Fluor euzymelinked immunosorbent assay;
Immunohistochemistry
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a method for postmortem diagnosis of the sudden death from hypersensitivity by detection of serum tryptase and IgE.Methods Venous blood samples were collected from 9 cases of sudden death from hypersensitivity,19 cases of death from other causes and 10 health living adult individuals.The serum levels of the mast cell tryptase(MCT)and IgE were detected with immunofluorescent enzyme assay and enzyme linked immunosorbent assay(ELISA)respectively.MCT in the lungs was detected by immunohistochemistical method.Results The serum level of both MCT and IgE in cases of sudden death from hypersensitivity were higher than those in health living adults and cases of death from other causes(P﹤0.01).However,there was significant difference(P﹥0.05)in the serum level of IgE and MCT between the health living individuals and cases of death from other cause.More cells containing MCT-positive granules were detected in the lung in cases of sudden death from hypersensitivity than in cases of death from other causes(P
