A simple and rapid modified-new method for DNA extraction of FTA bloodstains
- VernacularTitle:FTA-DNA直接提取法的研究与应用
- Author:
Jinzhi KUANG
;
Tonggang NIE
;
Zhi YANG
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
FTA bloodstain;
Direct extraction;
Automatic DNA
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and rapid new method for DNA extraction of FTA bloodstain samples.Methods Genomic DNA was extracted from FTA bloodstains of 1.2mm diameter by FTA-DNA direct extraction and FTA routine method respectively,and their genotypes were analyzed using ABI IdentifilerTM kit in 10?l and 25?l of reaction volume respectively.Results For 25?l of reaction volume,all DNA extracted by two different methods was successfully genotyped.For 10?l of reaction volume,however,the typing success rate of DNA extracted by FTA routine method was significantly lower than those by FTA-DNA direct extraction procedure.Using FTA routine method,the value of RFU ranged from 100 to 2000,and the peak imbalance result from preferential amplification of the smaller allele was a common phenomenon.Moreover,allelic dropout occurred in approximately nineteen percent of samples,and this was not obviously improved even if performed by automatic DNA workstation.However,using FTA-DNA direct extraction procedure,the typing results were similar to those in 25?l of reaction volume,and better results can be obtained using automatic DNA workstation.Conclusion The FTA-DNA direct extraction method is simple and rapid,and can be used to automatic establishment of DNA database with FTA bloodstains.
