Determination of promethazine and its metabolite in urine by solid——phase extraction with celite and UV derivative spectrophotometry
- VernacularTitle:尿中异丙嗪及其代谢物含量硅藻土萃取紫外导数光谱测定法
- Author:
Yuhong WU
;
Limei XING
;
Yingjian XU
- Publication Type:Journal Article
- Keywords:
Forensic toxicologcal analysis;
Promethazine and its metabolite;
Celite;
Solid-phase extraction;
UV derivative spectrophotometry;
Urine
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and rapid method for extraction and determination promethazine and its metabolite in urine.Methods The urine was added to celite column and the elutions was effected with dichloromethane.The eluat was evaporated on a water bath at 50℃.The dry residue was redissolved with 3.0ml H2SO4 solution of 0.05mol/L,and then zinc dust was added,in order to make it reaction on the water bath of 100℃ for 3min.The promethazine and its metabolite concentration were determined with the second derivative UV spectrum.Resvlts The extraction rate was 90% for both promethazine and its metabolite.The linear range of determination was 0.5～5.0?g/ml.Conclusion The method for determinating promethazine and its metabolite in urine is simple,convenience,and with high extraction rate.
