Predictable value of PCR in detecting bacterial DNAs for early diagnosis of jejunal anastomotic leakage and ileal anastomotic leakage in rats
- VernacularTitle:多聚酶链反应检测细菌DNA对大鼠空、回肠吻合口瘘的早期诊断价值
- Author:
Shanshan LEI
;
Tiegang LI
- Publication Type:Journal Article
- Keywords:
Stomas;
Intestinal Fistula/compl;
Polymerase Chain Reaction(PCR);
DNA,Bacterial/anal
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05),but the positive ratio of 16SrRNA genes expression in PB in Group C and Group E was significantly higher than that in group B and Group D respectively(P0.05).Conclusions(1)Detecting 16SrRNA genes from PB with PCR has certain significance for early diagnosis of jejunal anastomotic leakage and ileal anastomotic leakage,(2)PCR might be a useful tool for early diagnosis of jejunal anastomotic leakages and ileal anastomotic leakages by detecting lacZ genes or 16SrRNA genes from ascites.
