In vitro growth inhibition of gastric cancer cells (MFC) by siRNA targeting PKB
- VernacularTitle:蛋白激酶B siRNA转染对胃癌SGC-7901细胞生长增殖的影响
- Author:
Ceyao YANG
;
Bin ZHENG
;
Wenjing DUAN
;
Tide DUAN
;
Wei JIA
- Publication Type:Journal Article
- Keywords:
Stomach Neoplasms;
RNA interference;
Protein Kinase B;
Cell Cycle;
Cell Proliferation
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells.Akt2 expression was detected by RNAi technique,Akt2 protein level was detected by Western blot,and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry,SGC-7901 proliferation was measured by MTT method.Results After SGC-7901 cells transfected with Akt2 siRNA,the expression of protein level decreased obviously(P
