Effects of Yigu capsule on the level of cbf ?-1 mRNA in bone of ovariectomized osteoporosis rats
- VernacularTitle:益骨胶囊对去卵巢骨质疏松大鼠骨组织cbf ?-1mNRA表达的影响
- Author:
Bo LI
;
Ronghua ZHANG
- Publication Type:Journal Article
- Keywords:
Osteoporosis;
Ovariectomy;
Genes,cbf ?-1;
Yigu capsule
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To analyze the regulatory effect of Yigu capsule on core binding factor alpha 1 (cbf ?-1) gene expression in bone of ovariectomized osteoporosis (OP) rats. METHODS: Thirty-six 10-month old Sprague-Dawley female rats were randomized into three groups: sham-operated group, model group and drug group. After intervention by the corresponding methods, the femurs were collected, SYBR green Ⅰ fluorescence quantitative PCR technique was applied with the internal control of GAPDH according to the relative quantitative formula (2-Ct), the differentially expressed multiples between the model group or drug group and sham-operated group were calculated. RESULTS: Quantitative formula analysis showed that the level of cbf ?-1 gene expression in bone of model groups was decreased than that in sham-operated rats [compared with sham-operated group, P0.05). CONCLUSION: The results of this study show that cbf ?-1 gene expression in bone tissue of ovariectomized OP rat is decreased, and Yigu capsule increases the level of cbf ?-1 gene expression in bone of OP, indicating that Yigu capsule induces bone marrow mesenchymal stem cells into osteoblasts.
