Insulin protects endothelial progenitor cells against functional damage caused by high glucose
- VernacularTitle:高糖对内皮祖细胞功能的损害及胰岛素的影响
- Author:
Li ZHAO
;
Haichang WANG
;
Tao YIN
;
Kang CHENG
;
Feng GAO
- Publication Type:Journal Article
- Keywords:
Glucose;
Insulin;
Endothelial progenitor cells;
Arteriosclerosis
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the impact of various levels of glucose on endothelial progenitor cells (EPCs) proliferation, senescence, and nitric oxide (NO) secretion,and the effect of insulin under high glucose conditions.METHODS: Mononuclear cells were collected from rat bone marrow by density gradient centrifugation, cultured with medium 199, and identified to be EPCs at 7th day by flk-1 and AC133 double staining. EPCss were harvested and incubated with glucose (5, 10, 20, 40 mmol/L) or insulin (0.1, 1, 10, 100 nmol/L) under high glucose conditions for 24 h or 7 days. Proliferative capacity, senescence level and NO secretion (after 24 h of incubation) were subsequently determined.RESULTS: High glucose (40 mmol/L) markedly inhibited EPCs proliferation, accelerated EPCs senescence, and decreased NO production (all P
