Resistance and Detection of Resistant Genes of Multi-resistant Pseudomonas Aeruginosa
- VernacularTitle:多重耐药铜绿假单胞菌的耐药性及耐药基因检测
- Author:
Tingkun ZHAO
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
?-lactamase;
Antibiotics;
Drug resistance
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the resistance and prevalence of ?-lactamases in clinical isolates of P.aeruginosa and guide rational use of antibacterial agents.METHODS :MICs of 12 kinds of antibiotics to 70 P.aeruginosa isolates were tested by agar dilution.AmpC ?-lactamases and ESBLs were detected in 20 multi-drug P.aeruginosa strains by three-dimensional test.ESBLs genotypes were analyzed by PCR amplification and product sequencing.RESULTS:Imipenem,amikacin and ciprofloxacin had good activity against P.aeruginosa.Three-dimensional test showed that 2 strains possessed ESBLs,6 strains hyperexpressed AmpC ?-lactamases in 20 srains.PCR amplification and DNA sequencing showed that 4 strains with blaTEM,2 strains produced TEM-1,1 strain produced TEM-29,1 strain produced TEM ?-lactamase named as TEM-147 by GenBank with only one amino acid substitutionfrom TEM-29.CONCLUSIONS:Attention must be paid to the detection of ESBLs in P.aeruginosa which may cause clinical infections.Carbapenems,amikacin or ciprofloxacin can provide effective therapy for such infections.
