Determination of Two Components in Compound Chlortetracycline Hydrochloride Ointment by Dual Wavelength Spectrophotometry
- VernacularTitle:双波长分光光度法测定复方盐酸金霉素软膏中2主药的含量
- Author:
Liguang ZHENG
;
Guitian NIU
- Publication Type:Journal Article
- Keywords:
Dual wavelength spectrophotometry;
Chlortetracycline hydrochloride;
Tetracaine hydrochloride;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the contents of chlortetracycline hydrochloride and tetracaine hydrochloride in compound chlortetracycline hydrochloride ointment.METHODS:Dual wavelength spectrophotometry was used to determine the two components.The absorbance of the two components was determined at wavelength of 311 nm,368 nm and 387.5 nm,respectively;and the contents of which were computed as well.RESULTS:The linear ranges for chlortetracycline hydrochloride and tetracaine hydrochloride were 9.90～29.71 ?g?mL-1 and 5.99～17.98 ?g?mL-1(r=0.999 9),respectively,with average recovery rates at 99.37%(RSD=0.31%,n=9) and 98.31%(RSD=0.23%,n=9),respectively.CONCLUSION:The method is simple,rapid and accurate,and suitable for the determination of compound chlortetracycline hydrochloride ointment.
