Determination of Tanshinones in Shandantong Retard Tablets by HPLC
- VernacularTitle:HPLC法测定山丹酮缓释片中丹参酮类成分的含量
- Author:
Wenpeng YUAN
;
Linna LIU
- Publication Type:Journal Article
- Keywords:
Shandantong retard tablets;
HPLC;
Tanshinone;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for the determination of Tanshinone Ⅰ,Tanshinone ⅡA,Cryptotanshinone,and Dihydrotanshinone in Shandantong retard tablets.METHODS:The samples were separated on Lichrospher C18 column(250 mm?4.6 mm,5 ?m) with the mobile phase consisted of methanol-water(80∶20).The detection wavelength was set at 270 nm.RESULTS:The linear ranges of Tanshinone Ⅰ,Tanshinone ⅡA,Cryptotanshinone,and Dihydrotanshinone were 0.20～2.00 ?g,0.30～3.00 ?g,0.20～2.00 ?g,and 0.20～2.00 ?g,respectively.The average recoveries were 99.54%,98.89%,100.10%,and 99.44%,respectively with RSD at 0.40%,0.92%,1.30% and 1.53%(n=6),respectively.CONCLUSION:This method is simple,rapid and accurate,and suitable for the quality control of Shandantong retard tablets.
