Determination of Total Polysaccharides in Weile Granules by Phenol-sulfuric Acid Method
- VernacularTitle:苯酚-硫酸法测定围乐颗粒中总多糖的含量
- Author:
Hui LI
;
Jiabo LUO
- Publication Type:Journal Article
- Keywords:
Weile granules;
Total polysaccharides;
Phenol-sulfuric acid method;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish phenol-sulfuric acid method for the determination of the content of total polysaccharides in Weile granules.METHODS:The samples were deproteinized by Sevag method,colored by phenol-sulfuric acid method(water bath at 60 ℃ for 30 min),and the content of total polysaccharides in Weile granules was determined with Glucose as control.RESULTS:The linear range of Glucose was 20.47～102.36 ?g?mL-1(r=0.999 4),with an average recovery of 99.09%(RSD=0.92%,n=6).The content of total polysaccharides in Weile granules was 41.63%.CONCLUSION:This method was simple,accurate and reproducible,and suitable for quality control of Weile granules.
