Determination of Diosgenin in Bixiefenqing Pills by HPLC
- VernacularTitle:HPLC法测定萆薢分清丸中薯蓣皂苷元的含量
- Author:
Hongdi ZUO
;
Jiake BAO
;
Xiangjun MAO
- Publication Type:Journal Article
- Keywords:
Bixiefenqing pills;
Rhizoma Dioscoreae Hypoglaucae;
Diosgenin;
HPLC;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of Diosgenin in Bixiefenqing pills.METHODS:The samples were separated on DiamonsilTM C18(200 mm?4.6 mm,5 ?m)column.The mobile phase consists of acetonitrile-water(92∶8) at a flow rate of 1.0 mL?min-1.The detecting wavelength was set at 203 nm and the column temperature was set at 35 ℃.RESULTS:The linear range of Diosgenin was 0.115 36～1.038 24 ?g(r=0.999 9).The average recovery was 98.71%(RSD=1.92%,n=9).CONCLUSION:The method was proved to be simple and accurate,and suitable for the identification and quality control of Bixiefenqing pills.
