Reverse Effect of Baicalin/Baicalein on Antibiotic Resistance of Methicillin-resistant Staphylococcus Aureus
- VernacularTitle:黄芩苷/黄芩素对耐甲氧西林金黄色葡萄球菌抗药性的逆转作用研究
- Author:
Yongchuan CHEN
;
Linli XIE
;
Lirong XIONG
;
Min TANG
;
Peiyuan XIA
- Publication Type:Journal Article
- Keywords:
Baicalin;
Baicalein;
Oxacillin;
MRSA;
Reverse effect;
Penicillin-binding protein(PBP)
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the reverse effect of Baicalin/Baicalein on antibiotic resistance of methicillin-resistant staphylococcus aureus(MRSA) and its mechanism.METHODS:The synergetic antimicrobial effect of oxacillin and Baicalin/Baicalein against MRSA were detected by plating method,and the synergetic antimicrobial effect of oxacillin and Baicalin/Baicalein on growth density inhibition of MRSA was detected by spectrophotometry.The inhibitory effect of Baicalin/Baicalein on PBP2a synthesis was detected by PBP2a latex agglutination assay kit.RESULTS:The combination of Baicalin/Baicalein with oxacillin showed remarkable synergetic antimicrobial effect on clinical isolated MRSA.16 ?g?mL-1 of Baicalein obviously reversed the strong resistance of MRSA to oxacillin.Baicalin/Baicalein showed remarkable inhibitory effect on PBP2a production in MRSA.CONCLUSION:Baicalin/Baicalein can reverse MRSA's strong resistance to oxacillin by inhibiting PBP2a secretion in MRSA.Theoretically,this phenomenon offers a new approach for the treatment of infections caused by MRSA.
