Inhibitory Effect of Total Flavonoids of Ampelopsis Grossedentata on Human Hepatoma Carcinoma Cells in Vitro
- VernacularTitle:藤茶总黄酮体外抗人肝癌细胞作用研究
- Author:
Gang LI
;
Zuowen ZHENG
;
Yunli TANG
- Publication Type:Journal Article
- Keywords:
Total flavonoids of Ampelopsis grossedentata;
Human hepatoma BEL7404 cell;
Inhibitory effect in vitro
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effect of the total flavoniods of Ampelopsis grossedentata on human hepatoma carcinoma cells(HCC) in vitro.METHODS:The inhibitory effect of the total flavoniods of Ampelopsis grossedentata extract on human hepatoma carcinoma BEL7404 cells in vitro was analyzed by MTT,cell growth curve method and colony formation method,respectively.RESULTS:The total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells in vitro.MTT assay showed that the IC50 was 28.59 ?g?mL-1;the cell growth curve measurement showed that the total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells;the colony formation assay showed that the IC50 was 100% at a drug concentration of above 22.22 ?g?mL-1 and the IC50 was 58.05% at a drug concentration of 14.81 ?g?mL-1.CONCLUSION:The total flavonoids of Ampelopsis grossedentata have a remarkable inhibitory effects on the growth of BEL 7404 in vitro.
