Mechanism of protective effect of Shen-Fu injection(参附注射液)on renal failure induced by intestinal ischemia-reperfusion in rats
- VernacularTitle:参附注射液对大鼠肠缺血/再灌注期间肾保护作用机制的研究
- Author:
Yuhong HE
;
Chang CHEN
;
Zhongyuan XIA
- Publication Type:Journal Article
- Keywords:
kidney injury;
intestinal ischemia-reperfusion injury;
heme oxygenase;
inducible nitric oxide synthase;
Shen-Fu injection
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Shen-Fu injection(SFI,参附注射液) on expressions of heme oxygenase-1(HO-1) and inducible nitric oxide synthase(iNOS) in renal failure induced by intestinal ischemia-reperfusion injury(IRI) in rats and its possible mechanism in the protection of kidney.Methods The model of intestinal IRI was induced by clamping superior mesenteric artery(SMA) for 1 hour and then releasing the arterial clamp for 6 hours.Thirty-six male Wistar rats were randomly divided into three groups: IRI model group,SFI pretreatment group and sham operation group.In the SFI pretreatment group,10 ml/kg of SFI was pumped in at constant rate 30 minutes before the ischemia,the SMA was clumped for 1 hour and then released,while in the IRI model group,an equal volume of normal saline was pumped in continuously 30 minutes before the ischemia.The serum creatinine(SCr) and blood urea nitrogen(BUN) were observed respectively.Expressions and distributions of HO-1 and iNOS in the rat kidney tissue were detected by immunohistochemitry and morphometry computer image analysis.The histological changes of kidney were observed under light microscope.Results The expressions of HO-1 and iNOS were markedly higher,and the levels of SCr and BUN were also significantly higher in intestinal IRI model group than those in the sham operation group(all P
