Change of caspase-3 in rabbits after lung ischemia-reperfusion and the effect of puerarin(葛根素)
- VernacularTitle:葛根素对缺血/再灌注损伤兔肺组织天冬氨酸特异性半胱氨酸蛋白酶-3变化的影响
- Author:
Yixiao XU
;
Wantie WANG
;
Zhengjie XU
;
Xiaofeng JIN
;
Maolin HAO
- Publication Type:Journal Article
- Keywords:
lung ischemia-reperfusion injury;
apoptosis;
caspase-3;
puerarin
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of caspase-3 in rabbits after lung ischemia-reperfusion injury(LIRI) and the effect of puerarin(葛根素).Methods Thirty healthy rabbits used for unilateral lung ischemia-reperfusion model were randomly divided into 3 groups(each n=10): control group(C group),lung ischemia-reperfusion group(I/R group) and puerarin group.The activity of serum superoxide dismutase(SOD),the contents of serum malondialdehyde(MDA) and nitric oxide(NO),the wet to dry weight(W/D) ratio of lung tissue and the index of quantitative assessment of histological lung injury(IQA) were measured respectively in different groups;the pneumocyte apoptosis index(AI) was achieved by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL);caspase-3 protein and mRNA expression were studied by using in situ hybridization(ISH) and immunocytochemistry(IHC) techniques in the groups mentioned above.Results The activity of SOD and content of NO were significantly lower in I/R group than those in C group(both P
