Determination of Doxorubicin Hydrochloride Concentration in Human Plasma by RP-HPLC
- VernacularTitle:RP-HPLC法测定人血浆中盐酸多柔比星的浓度
- Author:
Yi LIU
;
Yuan BIAN
;
Yun YE
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Doxorubicin hydrochlorid;
Blood concentration
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for the determination of doxorubicin hydrochloride concentration in human plasma.METHODS:Tianhe Kromasil C18 was used as column,A solution of methenol-0.01 mol?L-1ammonium dihydrogen phosphate-acetic acid(30200.1) was used as the mobile phase at a flow rate of 1.0 mL?min-1.The detection of wavelength was set at 233 nm.The sample size was 20?L and the column temperature was 35℃.The internal standard was daunorubicin.RESULTS:The calibration curve was linear in the range from 0.25～20.16 ?g?mL-1(r=0.999 6);the average methodological recovery was 96.45%(RSD=2.16%).CONCLUSIONS:The method is rapid,accurate,reproducible and easy to use in clinical detection of blood concentration.
