Construction and appraise of SKP2 gene shRNA expression plasmid
- VernacularTitle:携带SKP2基因的shRNA表达质粒的构建与鉴定
- Author:
Jinshuai REN
;
Shi CHENG
;
Maomin SONG
- Publication Type:Journal Article
- Keywords:
Plasmids;
pSIREN-RetroQ Vector;
SKP2;
RNA Interference;
Plasmids;
Pancreatic Neoplasms/ther
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and appraise a series of shRNA eukaryotic expression plasmid which contains vary spots of human SKP2 gene.Methods Three pairs of hairpin-like oligonucleotide sequences specific for human SKP2 gene were designed and synthesized. They were cloned into the eukaryotic expression pSIREN-RetroQ plasmid. The recombinated plasmids were confirmed by PCR and sequencing.Results The shRNA sequences were constructed successfully, and could be inserted into the eukaryotic expression vector pSIREN-RetroQ.Conclusions Three shRNA transcripting plasmids pSIREN-RetroQ are successfully constructed, and form a basis for research in cancer gene target therapy against SKP2 gene.
