Effects of RUNX3 siRNA on the growth and drug sensitivity of SH-SY5Y cells
- VernacularTitle:RUNX3小干扰RNA对SH-SY5Y细胞生长和药物敏感性的作用
- Author:
Hongwei ZHANG
;
Lihua LIU
;
Chengzhong WANG
- Publication Type:Journal Article
- Keywords:
Neuroblastoma;
RUNX3;
RNA interference;
SH-SY5Y cells
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of RUNX3 gene on the growth and drug sensitivity of SH-SY5Y cells.METHODS: The siRNA plasmid of RUNX3 was constructed and transfected into SH-SY5Y cells.Stable transfectants were identified by RT-PCR and Western blotting.The growth curve,cell cycle distribution,drug sensitivity assay and accumulation of adriamycin in cells were detected by MTT assay and flow cytometry.The expressions of cyclin D1,CDK4,CDK6,p21,p27,Bcl-2,Bax,P-gp and MRP were analyzed by Western blotting.RESULTS: mU6pro-RUNX3 siRNA was successfully constructed and transfected into SH-SY5Y cells.Down-regulation of RUNX3 significantly promoted the cellular proliferation,inhibit the drug sensitivity and intracellular adriamycin accumulation of cells,compared with that in the controls(P
