Effect of RNA interference on HIF-2 in the renal cancer cell line 786-0
- VernacularTitle:RNA干扰缺氧诱导因子2对肾癌786-0细胞的影响
- Author:
Zhouhui PAN
;
Zhaodian CHEN
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor-2;
Kidney neoplasms;
RNA interference
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of RNA interference on hypoxia-inducible factor-2(HIF-2) in the renal cell cancer in vitro and in vivo.METHODS: HIF-2 RNAi was synthesized and inserted into RNA interference eukaryotic expression vector which was confirmed by sequencing.The vector was transfected into the renal cancer cell 786-0 and positive clone was selected by using G418.The HIF-2 expression was detected by RT-PCR and Western blotting method.The growth of cells was measured by MTT method.Nude mouse xenograft assays were also done.RESULTS: Compared with empty vector group and control group,the amounts of HIF-2 mRNA and protein expression were lower in the HIF-2 RNAi group,the difference was significant(P
