Change of endogenous hydrogen sulfide/cystathionine-?-lyase system in acute lung injury induced by LPS in rats
- VernacularTitle:内毒素性急性肺损伤大鼠内源性H_2S/CSE体系的变化
- Author:
Ping WANG
;
Jianxin ZHANG
;
Lanfang LI
;
Qinzeng ZHANG
;
Pule JIN
;
Cuimin DING
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Acute lung injury;
Hydrogen sulfide;
Interleukin-1;
Interleukin-10
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the changes of endogenous hydrogen sulfide/cystathionine-?-lyase(H2S/CSE) system while acute lung injury induced by LPS in rats.METHODS: Eighty rats were randomly divided into six groups(n=8): Ⅰ,control group;Ⅱ,LPS 1 h group;Ⅲ,LPS 3 h group;Ⅳ,LPS 6 h group;Ⅴ,LPS 9 h group;Ⅵ,LPS 12 h group.The ALI model of rats was prepared with LPS.The rats were respectively killed at 1,3,6,9 or 12 h after administration of LPS.The morphological changes of lung tissues were observed by light and electron microscope.The lung coefficient and the wet-to-dry weight ratio were measured.The contents of IL-1? and IL-10 in serum,the H2S level in plasma and the CSE activity in lung tissue were respectively detected.RESULTS: ⑴ In LPS 1 h group,the morphology,the lung coefficient,the wet-to-dry weight ratio,the H2S level and the CSE activity showed no changes compared with the control group.The contents of IL-1? and IL-10 were increased compared with the control group(IL-1?,P
