Effects of Glycosaminoglycan from Scallop Skirt on the Releasing of Vasoactive Substance from Endothelial Cells Injured by Hydrogen Peroxide
- VernacularTitle:扇贝裙边糖胺聚糖对过氧化氢损伤的内皮细胞释放血管活性物质的影响
- Author:
Ying GAO
- Publication Type:Journal Article
- Keywords:
Glycosaminoglycan from Scallop Skirt;
Endothelial cell;
H2O2;
Vasoactive substance
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observation the effects of Glycosaminoglycan from Scallop Skirt (SS-GAG) on the production of vasoactive substance in the endothelial cells injured by hydrogen peroxide(H2O2). METHODS: In H2O2-induced en-dothelial cell oxidative damage model group which was established with human umbilical vein endothelial cell (HUVEC, ECV304) cultured in vitro, SS-GAG groups (at different concentrations) and the normal group, levels of 6-keto-PGF1a, Thromboxane-B2 and Endothelin (ET) in the culture fluid were detected by radioimmunity assay and the content of nitric oxide was determined by nitrate reductase method. RESULTS: As compared with model group, SS-GAG (at different concentrations) significantly enhanced the levels of 6-keto-PGF1a and NO but significantly decreased levels of TXB2 and ET (P
