Determination of Chloramphenicol and Dapsone in Compound Chloramphenicol Cream by HPLC
- VernacularTitle:HPLC法测定复方氯霉素乳膏中主药的含量
- Author:
Hongmei LAN
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound chloramphenicol cream;
Chloramphenicol;
Dapsone;
Content
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream. METHODS: The analytical column was Hypersil ODS and the mobile phase consisted of 0.1%sodium heptanesulfonate solution (a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL)-acetonitrile (75∶25) at a flow rate of 1.0 mL?min-1. The detection wavelength was 272 nm and the sample size was 20 ?L. RESULTS: The linear ranges of chloramphenicol and dapsone were 25.59～153.54 ?g?mL-1 (r=0.999 9) and 12.57～75.42 ?g?mL-1 (r=0.999 9), respectively. The average recovery rates were 100.62% (RSD=0.92%) and 100.93% (RSD=1.22%), respectively. CONCLUSION: The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.
