Determination of Phospholipid Content in 5-FUR Prodrug Liposome
- VernacularTitle:5-氟尿嘧啶核苷前药脂质体中磷脂含量的测定
- Author:
Li ZHU
;
Lijun PAN
;
Chunmei HU
;
Junyi ZHAO
;
Chi WANG
- Publication Type:Journal Article
- Keywords:
5-FUR prodrug liposome;
Phospholipid;
Ammonium ferrothiocyanate spectrophometry
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the concentration of phospholipid in 5-FUR prodrug liposome. METHODS: The sample was fragmented by ethanol and extracted by chloroform. The concentration of phospholipid in 5-FUR prodrug liposome was determined by ammonium ferrothiocyarate spectrophometry at a wavelength 488 nm. RESULTS: The linear range of phospholipid was 0.01～0.10 mg?mL-1(r=0.999 6, n=3) with an average recovery rate of 99.66% (RSD=3.17%). CONCLUSION: This method is simple, convenient and sensitive, and it can be used for the quality control of 5-FUR prodrug liposome.
