Determination of Sirolimus and Probucol in Mixed-eluting Stents by RP-HPLC
- VernacularTitle:RP-HPLC法测定复合洗脱支架中西罗莫司和普罗布考的含量
- Author:
Lingling DU
- Publication Type:Journal Article
- Keywords:
Sirolimus;
Probucol;
Mixed-eluting stent;
RP-HPLC;
Content
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a RP-HPLC method for a simultaneously determination of the contents of sirolimus and probucol in the mixed-eluting stents. METHODS: Kromasil C18 column was used as an analytical column; the mobile phase consisted of acetonitrile and water which eluted by a gradient procedure at a flow rate of 1.0 mL?min-1. The detection wavelength was 277 nm; the column temperature was 55 ℃ and the sample size was 20 ?L. RESULTS: The calibration curves of sirolimus and probuol were linear in the range of 1.0～20.0 ?g?mL-1 (r=0.999 9). The average recoveries were 99.47%and 98.35%, respectively, with intra-day RSD and inter-day RSD all less than 5%. CONCLUSION: The method is simple, accurate and can be used to determine the contents of of sirolimus and probucol in the mixed-eluting stents.
