Determination of Tectoridin in Rhizoma Iridis Tectori by HPLC
- VernacularTitle:HPLC法测定川射干中鸢尾黄酮苷的含量
- Author:
Jia WANG
;
Chongjun YUAN
;
Yuguang YOU
;
Shuai CHENG
- Publication Type:Journal Article
- Keywords:
Rhizoma Iridis Tectori;
Tectoridin;
HPLC;
Determination content
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of Tectoridin in Rhizoma Iridis Tectori by HPLC. METHODS: The chromatographic separation was performed on Diamond ODS C18 column(150 mm?4.6 mm,5 ?m) with mobile phase consisted of MeOH-0.5% acetic acid(3∶7) at a flow rate of 0.8 mL?min-1. The detection wavelength was set at 265 nm.RESULTS: Good linear relationship was achieved over the concentration range of 0.079 2～0.396 0 ?g for tectoridin with an average recovery of 99.80%(RSD=1.09%,n=6). CONCLUSION: The method is sensitive, simple and accurate, and it can be used for the quality control of Rhizoma Iridis Tectori.
