Inhibition of invasiveness of human Hep-2 cell lines by heparanase antisense oligodeoxynucleotide
- VernacularTitle:乙酰肝素酶反义寡核苷酸对Hep-2细胞侵袭力抑制的研究
- Author:
Jiangang MA
;
Xiaoming LI
;
Xiuying LU
- Publication Type:Journal Article
- Keywords:
Heparin Lyas;
Oligodeoxyribonucleo tides, Antisense;
Laryngeal Neoplasms;
Neoplasms Invasiveness
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. METHODS HPSE AS- ODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized. After embedded by cation lipofectin, it was transfected into Hep-2 cells of human laryngocarcinoma. The expression of HPSE protein and HPSE mRNA in Hep-2 cell lines were detected by flow cytometry and RT- PCR. Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. RESULTS The HPSE protein and HPSE mRNA expression and invasiveness of human Hep-2 cells treated with AS- ODN of different concentrations were significantly decreased as the AS-ODN concentration increasing. There was a significantly difference between control group and each group of AS-ODN respectively (P
