Inhibiting the expression of proinflammatory factors in human nasal epithelial cells by dexamethasone
- VernacularTitle:地塞米松对人鼻黏膜上皮细胞前炎性因子表达的抑制作用
- Author:
Linhong YANG
;
Zhen DONG
- Publication Type:Journal Article
- Keywords:
Epithelial cells;
Lipopolysaccharide;
Interleukin-1;
Hypoxia Inducible Factor 1, alpha Subunit;
Vascular Endothelial Growth Factor A;
Dexamethasone
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the inhibition of the expression of HIF-1? and VEGF induced by LPS in primary human nasal epithelial cell (HNEC) by dexamethasone. METHODS Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of LPS 100 ng/ml, IL-1? 20 ng/ml, LPS 100 ng/ml +dexamethasone 13 ng/ml and IL-1? 20 ng/ml+ dexamethasone 13 ng/ml for 3h, 6h and 9h respectively. The expression of HIF-1?, VEGF protein and mRNA derived from epithelial cells was detected by immunocytochemistry and situ hybridization. RESULTS ①The expression of HIF-1? and VEGF increased under the stimulus of LPS and IL-1? with the increasing of time and concentration, especially under LPS 100 ng/ml for 6h (P
