Determination of Epirubicin in Human Plasma by RP-HPLC
- VernacularTitle:RP-HPLC法测定人血浆中盐酸表柔比星的浓度
- Author:
Lijie YANG
;
Tian QI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Epirubicin;
Serum drug concentration
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of epirubicin in human plasma by RP-HPLC.METHODS:The chromatographic separation was performed on YWG C18 column.The mobile phase consisted of water-acetonitrile(12∶7,with pH adjusted to 3.90?0.05 using 85% phosophoric acid)at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 254 nm.RESULTS:The linear range of epirubicin was 0.12～4.80 mg?L-1(r=0.998 4)with the lowest detective range of 0.01 mg?L-1.The intraday RSD was less than 4.40% and the inter-day RSD was less than 5.60%,and the average recovery rate was 99.40%(RSD=4.02%).The sample solution was stable within 12 h under room temperature.CONCLUSION:The method is easy,precise and reproducible to operate and the result of the determination is accurate.
