Determination of Serum Concentrations of Phenbarbital,Phenytoin Sodium and Carbamazepine by MEKC
- VernacularTitle:胶束电动毛细管色谱法同时测定人血清中3种抗癫痫药的浓度
- Author:
Yinghua LIU
;
Xiaohua YANG
- Publication Type:Journal Article
- Keywords:
Micellar electrokinetic capillary chromatograph;
Phenbarbital;
Phenytoin sodium;
Carbamazepine;
Serum concentration
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine serum concentrations of phenbarbital(PB),phenytoin sodium(PHT),carbamazepine(CBZ)by micellar electrokinetic capillary chromatograph(MEKC).METHODS:The determination was performed on elastic quartz column(37 cm?50 ?m)with barbital as internal standard.The buffer solution consisted of 10 mmol?L-1 phosphate sodium buffer-30 mmol?L-1 SDS sodium(micellar phase)-15% methanol at a constant voltage of 18 kV.The detection wavelength was set at 210 nm.RESULTS:The linear concentration ranges of PB and PHT were 2.5～80 ?g?mL-1,with detective concentration of 0.1 ?g?mL-1;the linear range and detective concentration of CBZ were 1～32 ?g?mL-1 and 0.2 ?g?mL-1,respectively.The average recoveries of the PB,PHT,and CBZ were 99.90%,99.91%,and 99.42%,respectively.CONCLUSION:The method is rapid,simple,accurate,sensitive and reproducible,and suitable for clinical monitoring of drug level and pharmaceutical study.
