The application of MiniFiler~(TM) kit in LCN-STR genotyping
- VernacularTitle:MiniFiler~(TM)试剂盒在LCN-STR分型中的应用
- Author:
Weiwei WU
;
Xiaoting ZHENG
;
Honglei HAO
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
Low copy number(LCN);
Mini-STR;
Sensitivity
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application value of MiniFilerTM kit in LCN-STR genotyping in forensic science.Methods Compared the testing results of MiniFilerTM and IdentifilerTM kits in 49 general quantity biological samples and 39 LCN-biological samples,including blood stains,sperm stains,bones,and so on.Meanwhile the sensitivity of these two kits were also compared.Results Full concordance between IdentifilerTM and MiniFilerTM kits was observed in all of 49 general quantity biological samples.For 39 LCN-biological samples,only 22 samples could be genotyped in partial loci and 17 samples negative with the IdentifilerTM kit,while 30 samples could be genotyped in all loci,5 samples in partial loci and 4 samples negative with the MiniFilerTM kit.Therefore,there was a higher success rate for LCN-biological samples typing with MiniFilerTM kit than with IdentifilerTM kit.In addition,the sensitivity of the MiniFilerTMkit was also a little higer than the IdentifilerTMkit.Conclusion The results demonstrated that the MiniFilerTM kit can markedly improve the typing success rate of LCN-biological samples,and is suitable for analyzing difficult biological samples in forensic practice.
