Effect of shock lymph on apoptosis relative gene expression in pulmonary micro-vascular endothelial cells
- VernacularTitle:休克淋巴液对大鼠肺微血管内皮细胞凋亡相关基因表达的影响
- Author:
Chunyu NIU
;
Zigang ZHAO
;
Ruihua CHEN
;
Jing ZHANG
;
Yuping ZHANG
;
Yankai LIU
- Publication Type:Journal Article
- Keywords:
Shock;
Lymph;
Lung;
Endothelial cells;
Apoptosis;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of shock lymph on apoptosis relative gene expressions of pulmonary micro-vascular endothelial cells(PMVECs),and explore its mechanism.METHODS: The model of severe hemorrhagic shock was established by maintaining the blood pressure of rats in the condition of sepsis,mesentery lymph and shock portal vein blood was taken out.As control,mesentery lymph,portal vein blood of normal rats was taken out.The primary PMVECs of passages 3 were treated by different treatment factors,respectively.The apoptosis rate was analyzed by flow cytometry,and the expressions of relative genes of apoptosis such as fas,fas L,bcl-2 and bax were detected by RT-PCR.RESULTS: The apoptosis rate of PMVECs was 9.86%?3.24% after exposed to shock lymph at the final concentration of 4% for 4 hours and significantly higher than that in control(P
