PKC-? is involved in the regulation of VEGF mRNA expression in SDF-1 induced U937 cells
- VernacularTitle:PKC-?参与调节基质细胞来源的因子-1诱导的人U937细胞VEGF mRNA表达
- Author:
Pengcheng LI
;
Qian XU
;
Yanhua HU
- Publication Type:Journal Article
- Keywords:
Protein kinase C;
shRNA;
Stromal cell-derived factor-1;
Vascular endothelial growth factors;
U937 cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of protein kinase C-?(PKC-?) in U937 cell line inhibited by short hairpin RNA(shRNA) on the transcription of vascular endothelial growth factor(VEGF) mRNA induced by stromal cell-derived factor-1(SDF-1).METHODS: 64 bp reverse repeated motifs of PKC-? target sequence were synthesized and inserted into the plasmid to construct the plasmid expressing shRNA-PKC-?(pSIRENp) and the pSIRENp plasmid was transfected into U937 cell line.The expression of PKC-? and VEGF mRNA was detected by RT-PCR.RESULTS: The recombinant plasmid pSIRENp was successfully constructed and it nearly completely suppressed the PKC-? expression in U937 cell line.After transfection,both basical and VEGF mRNA induced by SDF-1 significantly reduced compared to control.CONCLUSION: The results shows that the short hairpin RNA of PKC? efficiently reduces its expression in U937 cells and PKC-? may be involved in the regulation of VEGF mRNA expression.
