Activation of NF-?B and expression of ICAM-1 and COX-2 genes in rat neointimal formation
- VernacularTitle:大鼠血管新生内膜形成过程中NF-?B的活化和ICAM-1及COX-2表达的变化
- Author:
Mei HAN
;
Jinkun WEN
;
Yueping LIU
;
Wei SONG
- Publication Type:Journal Article
- Keywords:
Endothelium,vascular;
NF-kappa B;
Tunica intima;
Rats;
Intercellular adhesion molecule-1;
Clooxygenase-2
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between NF-?B activation and intimal hyperplasia.METHODS: The protein extract from aorta was used to detect the expression of NF-?B p65,I?B?,ICAM-1 and COX-2 by Western blotting,and detection of threonine phosphorylation of p65 was performed by immunoprecipitation analysis.RESULTS: The level of p65 reached a maximum level at day 7,and then decreased significantly,which was higher than that in the control,in both cellular extract and nuclear extract at day 21 after balloon injury.The p65 level in cytoplasm had no obvious changes at different time points after balloon injury.The threonine phosphorylation of NF-?B p65 was negative correlated with the nuclear translocation of NF-?B.I?B? level showed a 15% decrease at day 1 after balloon injury,compared with control group,then returned at day 14,and reached to normal level at day 21.However,the expression of ICAM-1 and COX-2 was maximal at day 14,and then declined but higher than that in control group at day 21 after balloon injury.CONCLUSION: The activation of NF-?B p65 and the expression of ICAM-1 and COX-2 are parallel to the neointimal thickening after balloon injury.
