Effect of nicotine against apoptosis of rat cortical neurons induced by colchicines
- VernacularTitle:烟碱拮抗秋水仙碱诱导的乳大鼠大脑皮质神经元凋亡
- Author:
Xiaohui HUANG
;
Xiaonan ZHU
;
Qin WANG
;
Ruzhu CHEN
;
Xuelan WANG
- Publication Type:Journal Article
- Keywords:
Nicotine;
Protein-serine-threonine kinases;
Cerebral cortex;
Neurons;
Colchicine;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the mechanism of nicotine against the apoptosis induced by colchicines in rat cortical neurons.METHODS: Cortical neurons were cultured from newborn Sprague-Dawley(SD) rats(less than 12 h).The rate of apoptosis was measured by Hoechst33258 fluorescence staining in the neurons,and the activity of Akt473 was analyzed by assay kit Akt473.RESULTS: The apoptosis of cortical neurons can be induced by 0.1 ?mol/L colchicine.The phosphorlation of Akt 473 decreased significantly(1/3 times of the control group,P
