Content Determination of Cholesterol Esters in Foam Cells Derived from RAW264.7 by HPLC
- VernacularTitle:HPLC法测定RAW264?7源性泡沫细胞中胆固醇酯的含量
- Author:
Xiaoqing YANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Pravastatin;
Cholesterol esters;
Foam cells
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of levels of intracellular cholesterol and cholesterol esters in foam cells derived from RAW264.7 murine macrophages so as to obtain a precise measure of foam cells and provide a technical platform for the study of the intracellular cholesterol content change.METHODS:HPLC method was applied to determine the content of cholesterol esters in the foam cells treated with OX-LDL and different concentration of pravastatin.RESULTS:OX-LDL could significantly enhance cellular intake of lipids(P
