Experimental Study on Apoptosis Induced by Ethanol Extracts of Solanum Lyratum Thunb in Human Lung Cancer SPC-A-1 Cells
- VernacularTitle:白英乙醇提取物诱导人肺癌SPC-A-1细胞凋亡的实验研究
- Author:
Xing WEI
- Publication Type:Journal Article
- Keywords:
Solanum lyratum Thunb;
Ethanol extracts;
Lung cancer SPC-A-1 cells;
Apoptosis;
fas;
caspase-3
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of ethanol extracts of Solanum lyratum Thunb(ST) on induction of apoptosis and the expression of apoptosis associated genes fas and caspase-3 in human lung cancer SPC-A-1 cells.METHODS:Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group,ethanol extracts of ST treated groups(2.5、5、10 mg?L-1)and the positive control group(cisplatin).After treatment with drug for 48 h,the proliferation inhibitory rate was evaluated by MTT assay,induction of cell apoptosis rate was determined by TUNEL method,the expression of fas and caspase-3 mRNA was detected by semi-quantitive RT-PCR.RESULTS:Compared with control group,the inhibitory rate was increased obviously(P
