Determination of Main Component Content in Compound Dyclonine Ointment by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方达克罗宁软膏中主药的含量
- Author:
Yuelong YANG
- Publication Type:Journal Article
- Keywords:
Compound dyclonine ointment;
RP-HPLC;
Dyclonine;
Content determination
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of dyclonine hydrochloride in compound dyclonine ointment by RP-HPLC.METHODS:The chromatographic separation was performed on Lanbo C18 with mobile phase consisted of acetonitrile-water-glacial acetic acid-triethylamine(55:43:1:0.5) at a flow rate of 1.0 mL?min-1.The sample size was 20 ?L and the wavelength was set at 279 nm.RESULTS:The linear range of dyclonine hydrochloride was 10.43～62.58 ?g?mL-1(r=0.999 7) with an average recovery rate of 98.52%(RSD=0.93%).CONCLUSION:The method is simple,rapid and effective,and it can be used for the quality control of compound dyclonine ointment.
