Quantitative Determination of Scutellarin in Kang’aiping Pills by HPLC
- VernacularTitle:HPLC法测定抗癌平丸中野黄芩苷的含量
- Author:
Ling PAN
- Publication Type:Journal Article
- Keywords:
Kang’aiping pills;
Scutellarin;
HPLC;
Quantitative determination
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of Scutellarin content in Kang’aiping pills. METHODS: The chromatographic separation was performed on DiamondTM C18 column (250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water-acetic acid glacial(20∶80∶1) at a flow rate of 1.0 mL?min-1. The column temperature was set at 45 ℃ and the detection wavelength was set at 335 nm. RESULTS: The linear range of scutellarin was 0.267 5～2.140 0 ?g(r=0.999 9) and the recovery rate was 98.33%(RSD=1.34%,n=6).CONCLUSION: This method is simple, practicable, accurate and reliable, and it can be used for the quality control of Kang’aiping pills.
