Determination of Chlorogenic Acid in Pneumonia Mixture by RP-HPLC
- VernacularTitle:RP-HPLC法测定肺炎合剂中绿原酸的含量
- Author:
Quanmei ZHANG
;
Bing LIU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Chlorogenic acid;
Pneumonia mixture;
Content determination
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of Chlorogenic acid in Pneumonia mixture. METHODS: The separation of samples was performed on a column of Spheri-5 RP-C18 (250 mm?4.6 mm,5 ?m). The mobile phase consisted of acetonitrile-0.4% phosphate (8.5∶91.5) with a flow rate of 1.0 mL?min-1.The detection wavelength was set at 328 nm. RESULTS: The calibration curve was linear in the range of 5.00～100.00 ?g?mL-1 for Chlorogenic acid(r=0.999 9).The average recovery was 98.15%,(RSD=3.68%,n=6).CONCLUSION: The method was rapid, simple and accurate, and it can be used for the quality control of Pneumonia mixture.
