Determination of Indirubin Content in Banlangen Granules by HPLC
- VernacularTitle:HPLC法测定板蓝根颗粒中靛玉红的含量
- Author:
Yizhou YAN
;
Pinwen ZOU
- Publication Type:Journal Article
- Keywords:
HPLC;
Banlangen granules;
Indirubin;
Content determination
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the content of Indirubin in Banlangen granules. METHODS: The choromatographic separation was performed on C18 column (250 mm?4.6 mm,5 ?m) with column temperature set at 30 ℃. The mobile phase consisted of methanol-water(76∶24) at a flow rate of 1.0 mL?min-1.The dete-ction wavelength was set at 289 nm.RESULTS:The calibration curve of Indirubin was linear in the range of 0.073 5～0.612 5 ?g (r=0.999 9) and the average recovery was 98.78%(RSD=0.74%,n=9).CONCLUISON: The method is rapid and accurate, and it can be used for quality control of Banlangen granules.
