Effects of panax notoginseng saponins(三七总皂苷)on activity of lung nuclear factor-?B and acute lung injury in rats with acute necrotizing pancreatitis
- VernacularTitle:三七总皂苷对急性坏死性胰腺炎大鼠肺组织核转录因子-?B活性及肺损伤的影响
- Author:
Mingwei LIU
;
Mingshun AN
- Publication Type:Journal Article
- Keywords:
acute necrotizing pancreatitis;
rat;
nuclear factor-?B;
panax notoginseng saponins
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether panax notoginseng saponins(三七总皂苷,PNS) can inhibit the activity of lung nuclear factor-?B(NF-?B) and ameliorate the lung pathologic injury in rats with acute necrotizing pancreatitis(ANP).Methods Thirty Sprague-Dawley(SD) rats were randomly divided into three groups: sham operation group(group sham),ANP group(group ANP),PNS preconditioning group(group PNS).There were 10 rats in each group.Rat ANP model was induced by retrograde injection of 5% sodium taurocholate to pancreatic duct(1 ml/kg).The rats in the PNS preconditioning group were given by intraperitoneal injection of 50 g/L PNS(1 ml/kg) one hour before model establishment,and those in the other two groups were given 0.9% physiological saline(1 ml/kg) one hour before the operation.Put the rat in each group to death at 6 hours after the respective management was made,and the pulmonary tissues were taken to detect the activity of NF?B by immunohistochemistry and the wet/dry weight ratio of the lung tissue was measured.Results At 6 hours after the model was established in the ANP group,the NF-?B positive cell number(70%-100% positive cells,7 vs.0) and the positive unit((72.52?7.63)PU vs.(33.09?4.75)PU) in the lung tissue were remarkably increased in comparisons with those in the sham group(both P
