The effect of expression of RNA interference targeting PKB mediated by nanoparticles on intimal hyperplasia in vein graft of rats
- VernacularTitle:RNA干扰沉默蛋白激酶B基因表达对移植静脉内膜增生的影响
- Author:
Weidong YU
;
Xinhua HU
;
Zhishen ZHANG
;
Tiemin LI
;
Jun YANG
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Protein Kinase B;
shRNA;
Intimal Hyperplasia;
Nanoparticle
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of expression of RNA interference targeting protein kinase B(PKB) gene transfection mediated by nanoparticles(NP) on intimal hyperplasia(IH) in vein grafts of rats.Methods Nanoparticle PKB short hairpin RNA(shRNA) gene complex was prepared with PLGA and PVA.Autogenous vein graft model was established in 72 rats by transplanting internal jugular vein to carotid artery.The models were randomly divided into 3 groups:(1) PKB shRNA group: PKB-shRNA gene mediated by NP were transfected into the veins before anastomosis.(2) Empty vector group: the veins were transfected by empty vector mediated by NP.(3) Control group(no transfection).The grafted veins were harvested 3,7,14 and 28 days after the operation respectively.The mRNA and protein expression of PKB were determined by Northern blot and Western blot.IH was observed by HE and Verhoeff stain.The presence of apoptotic VSMC was detected by TUNEL stain.Results Compared to empty vector group and control group,PKB shRNA group had less expression of mRNA and protein of PKB gene(P
